genomic libraries การใช้

• Construction of a genomic library involves creating many recombinant DNA molecules.
• Cosmids can be used to build genomic libraries.
• Genomic libraries are commonly used for sequencing applications.
• Genomic libraries are often used in combination with whole genome shotgun sequencing for this reason.
• Determining expression and developing genomic libraries are some applications for which the pBS recombinants can be used.
• A genomic library is a set of clones that together represents the entire genome of a given organism.
• Genes can be isolated through genomic libraries and used on human cell lines or animal models to further research.
• A high resolution map can be created by sequencing both ends of inserts from several clones in a genomic library.
• Positional cloning typically involves the isolation of partially overlapping DNA segments from genomic libraries to progress along the chromosome toward a specific gene.
• After a genomic library is constructed with a viral vector, such as lambda phage, the titer of the library can be determined.
• Human genomic libraries were screen under low-stringency conditions using probes from animal retroviruses, allowing the isolation and characterization of multiple, though defective, proviruses, that represented various families.
• A similar method can be used to titer genomic libraries made with non-viral vectors, such as plasmids and ligation of the library can be used to transform E . coli.
• The number of clones that constitute a genomic library depends on ( 1 ) the size of the genome in question and ( 2 ) the insert size tolerated by the particular cloning vector system.
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• This sequence of cDNA was used to find the remaining DNA sequences comprising the Factor IX gene by searching the DNA in the X chromosome : A genomic library from a human XXXX chromosome was prepared . . . and screen [ ed ] with a Factor IX cDNA probe.